Cleavage of Lambda DNA with Eco RI: An Introduction to Restriction Enzymes

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Price: $230 + GST
Model Number: 212


For 10 restriction digestions and 5 gels.

Complete in 90 minutes.

The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (5 distinct sites; 2 are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analyzed by agarose gel electrophoresis.

Kit includes:

  • Instructions
  • Lambda DNA
  • Dryzymes®
  • Reconstitution buffer
  • Restriction enzyme reaction buffer
  • Enzyme grade water
  • Standard DNA Fragments
  • Various solutions and buffers
  • Agarose powder
  • FlashBlue™ Stain

Requirements:

  • Electrophoresis apparatus
  • Power supply
  • Automatic pipette with tips
  • Waterbath
  • Ice