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Products >> Experiments >> DNA Electrophoresis >> Ready-to-Load™ DNA Samples >> 135- Using CRISPR To Treat Cystic Fibrosis
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In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.
Group Size:
For 6 Gels
Time Required:
Complete in 45 minutes
Kit Includes:
Instructions, Ready-to-Load™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain.
All You Need:
DNA Electrophoresis Apparatus, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.
Storage:
Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended.
Also Available: DNA Samples Only
Cat. # 135-A (for 6 gels) @ $125 +GST
Cat. # 135-B (for 12 gels) @ $249 +GST
Cat. # 135-C (for 24 gels) @ $498 +GST