Simulation of HIV Detection by Western Blot
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Price: $250 + GST
Model Number: 275
For 6 Blots.
Electrophoresis 45 minutes.
Blot overnight
Detection 25 minutes.
The second assay used to confirm a positive HIV ELISA result is the Western Blot. Students separate protein samples from hypothetical patients on agarose gels, transfer the samples to a membrane and detect the simulated HIV proteins. This kit is an introductory level experiment. For a comprehensive advanced course, we recommend Cat. #317.
Kit includes:
- Instructions
- Samples
- Standard molecular weight markers
- Protein agarose
- Various buffers and reagents
- PVDF membrane
- Filter paper
- Stain
- 1 ml pipette
Requirements:
- Electrophoresis apparatus
- Power supply
- Automatic micropipettes with tips
- Incubation oven
- Shaker platform (optional)
- Micro test tubes
- Isopropanol
- Glacial acetic acid
