Southern Blot Analysis

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Price: $189 + GST
Model Number: 207


For 5 Lab Groups.

Electrophoresis 45 minutes.

Blotting overnight Staining & destaining 10 minutes.

This experiment introduces your students to Southern blotting as a tool for “DNA Fingerprinting” in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualized by staining.

Kit includes:

  • Instructions
  • DNA samples for electrophoresis
  • Practice gel loading solution
  • UltraSpec-Agarose™
  • Electrophoresis buffer
  • Pipettes
  • 5 pre-cut nylon membranes
  • 5 pre-cut blotting filter papers
  • Blue-Blot DNA Stain™.

Requirements:

  • Electrophoresis apparatus
  • Power supply
  • 65° C Waterbath
  • DNA visualization system
  • Automatic micropipettes
  • NaCl, NaOH, concentrated HCl