Southern Blot Analysis
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Price: $238 + GST
Model Number: 207
For 5 Lab Groups.
Electrophoresis 45 minutes.
Blotting overnight Staining & destaining 10 minutes.
This experiment introduces your students to Southern blotting as a tool for “DNA Fingerprinting” in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualized by staining.
Kit includes:
- Instructions
- DNA samples for electrophoresis
- Practice gel loading solution
- UltraSpec-Agarose™
- Electrophoresis buffer
- Pipettes
- 5 pre-cut nylon membranes
- 5 pre-cut blotting filter papers
- Blue-Blot DNA Stain™.
Requirements:
- Electrophoresis apparatus
- Power supply
- 65° C Waterbath
- DNA visualization system
- Automatic micropipettes
- NaCl, NaOH, concentrated HCl
