303- Exploring Biotechnology with Green Fluorescent Protein (GFP)
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Price: $410 + GST
Model Number: 303
For 6 experiments with 4 modules each.
Transformation 45 minutes.
Plating 5 minutes.
Incubation overnight.
Transformation efficiency 15 minutes.
Packing & running the column 45 minutes.
Optional electrophoresis 60 minutes.
Staining/destaining 30-120 minutes.
Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the green fluorescent protein (GFP). The transformed cells are then grown and the GFP is purified by column chromatography. Finally, the purity of the protein fractions are analyzed by SDS polyacrylamide electrophoresis.
Kit includes:
- Instructions
- Transformation cells
- Plasmid DNA for GFP
- IPTG
- Ampicillin antibiotic
- Calcium chloride
- ReadyPour ™ luria broth agar
- Luria broth media for recovery
- Petri dishes
- Pipets
- Calibrated transfer pipets
- Inoculating loops
- Micro test tubes with attached caps
- Toothpicks
- Dry matrix for columns
- Chromatography columns
- Green and blue fluorescent protein extracts
- Elution buffer
- Protein molecular weight standards
- Protein denaturation solution
- Glycerol solution
- Tris-Glycine-SDS buffer
- Stains
Requirements:
- Incubation oven
- Waterbath
- Automatic micropipette and tips
- Ice
- Long wave UV light (hand-held is fine)
- Ring stand and clamps
- Vertical gel electrophoresis apparatus and power supply
- 3 Polyacrylamide Gels (12%)
- Glacial acetic acid
- Methanol
