Water Quality Testing III: Multiplex PCR Testing of Water Contaminants
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Price: $410 + GST
Model Number: 953
For 25 Students
Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
Extraction 60 mins
PCR Setup 10 mins
PCR 2 hours
Electrophoresis 60 mins
Staining 5 mins or overnight
Kit includes: instructions, PCR EdvoBeads™, Universal DNA Buffer, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™ (Complete PCR Control), TE Buffer, Proteinase K, Potassium Acetate, DNA Extraction Buffer, BactoBeads™, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes.
All you need: DNA Electrophoresis, Thermal Cycler, Micropipets: 5-50 µl, UV or Blue Light Transilluminator, Water Bath, Microcentrifuge, Microwave or Hot plate, & Isopropanol.
Storage: Some Components Require Refrigerator or Freezer Storage Upon Receipt.
