Isolation of E. coli Chromosomal DNA

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Price: $250 + GST
Model Number: 203


For 20 DNA isolations and 5 gels.

Isolation of Plasmid DNA 60 min. Electrophoresis 45 min.

Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA Extraction LyphoCells™ and reagents for isolating chromosomal DNA from  E. coli. After spooling from solution, the DNA can be dissolved and analyzed by agarose gel electrophoresis as an optional lab extension activity.

Kit includes:

  • Instructions
  • LyphoCells™
  • Required solutions and buffers
  • Agarose powder
  • FlashBlue™ Stain.

Requirements:

  • Waterbath
  • 95-100% isopropanol.

For optional electrophoresis:

  • Electrophoresis apparatus
  • Power supply
  • Automatic micropipet with tips