Water Quality Testing III: Multiplex PCR Testing of Water Contaminants
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Price: $410 + GST
Model Number: 953
For 25 Students
Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
Time Required:
Extraction - 60 minutes
PCR Setup - 10 minutes
PCR - 2 hours
Electrophoresis - 60 minutes
Staining - 5 mins or overnight
Kit includes:
- Instructions, PCR EdvoBeads™
- Universal DNA Buffer
- LyphoPrimer™ Mix
- EdvoQuick™ DNA Ladder
- LyphoControl™ (Complete PCR Control)
- TE Buffer
- Proteinase K
- BactoBeads™
- UltraSpec-Agarose™
- Electrophoresis Buffer (50X)
- SYBR® Safe Stain
- FlashBlue™ Stain
- Microcentrifuge/PCR Tubes.
Requirements:
- Micropipettes to measure between 5 and 50 μl
- Tips
- Waterbath
- Microcentrifuge
- Thermal cycler
- Electrophoresis apparatus
- Power supply
- UV transilluminator or TruBlu Blue Light.
