Isolation of Plant Mitochondria & Chloroplasts

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Price: $219 + GST
Model Number: 910


For 6 Groups.

Requires approx 5.5 hours for completion over 3 lab sessions.

In this two-part experiment, your students will explore the techniques used to isolate plant organelles. Two cell organelles are isolated from pea seedlings by differential centrifugation. First, students identify mitochondria by the enzyme activity of cytochrome c oxidase. Then, chloroplasts are isolated and identified under the microscope.

Kit includes:

  • Instructions
  • Chloroplast isolation reagents
  • Mitochondrial DNA isolation reagents
  • Transfer pipettes
  • Pea seeds.

Requirements:

  • Microscope and slides
  • Spectrophotometer
  • Blender
  • Waterbath
  • Clinical or high speed centrifuge (10,000 x g)
  • Automatic micropipettes & tips