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For 6 Groups.
Requires approx 5.5 hours for completion over 3 lab sessions.
In this two-part experiment, your students will explore the techniques used to isolate plant organelles. Two cell organelles are isolated from pea seedlings by differential centrifugation. First, students identify mitochondria by the enzyme activity of cytochrome c oxidase. Then, chloroplasts are isolated and identified under the microscope.