In Search of the Alcohol Gene

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Price: $399 + GST
Model Number: 346


For 6 Groups

PCR 2 hrs. 15 minutes.

Restriction Enzyme Digestion 65 minutes.

Electrophoresis 55 minutes.

Staining 15 minutes.

The first step in alcohol metabolism is the conversion of alcohol to acetaldehyde by Alcohol Dehydrogenase (ADH). The rate of alcohol metabolism is dependent on several environmental and genetic factors (body weight, food intake, gender, genetics). There are several variants of the ADH gene that differ based on polymorphisms that produce enzymes having different rates of alcohol metabolism. Different polymorphic genes can be identified using PCR amplification followed by restriction enzyme digestion and analysis by agarose gel electrophoresis. In this experiment, students will identify a simulated ADH polymorphic gene sequence by amplification and digestion of DNA. Students will perform PCR amplification reactions of several DNA samples. After checking the amplification products on an agarose gel, the remaining sample will be digested with a restriction enzyme. When the digested samples are analyzed on an agarose gel, students will identify different polymorphic genes.

Kit includes:

  • Instructions
  • DNA template
  • Primer mix
  • PCR reaction beads
  • RNAse-free water
  • DNA size ladder
  • Eco RI restriction enzyme
  • Restriction enzyme reaction & dilution buffers
  • Agarose
  • Electrophoresis buffer
  • InstaStain® Ethidium Bromide gel stain

Requirements:

  • Thermal cycler
  • Electrophoresis apparatus
  • Power supply
  • Automatic micropipette
  • Waterbath
  • UV transilluminator