Tomorrow's BioScience Education Today
Question, comment or just feedback we want to know. Maybe you have question on what may compliment this product? We have the answers so ask the questions. We look forward to hearing from you.
For 6 Groups
PCR 2 hrs. 15 minutes.
Restriction Enzyme Digestion 65 minutes.
Electrophoresis 55 minutes.
Staining 15 minutes.
The first step in alcohol metabolism is the conversion of alcohol to acetaldehyde by Alcohol Dehydrogenase (ADH). The rate of alcohol metabolism is dependent on several environmental and genetic factors (body weight, food intake, gender, genetics). There are several variants of the ADH gene that differ based on polymorphisms that produce enzymes having different rates of alcohol metabolism. Different polymorphic genes can be identified using PCR amplification followed by restriction enzyme digestion and analysis by agarose gel electrophoresis. In this experiment, students will identify a simulated ADH polymorphic gene sequence by amplification and digestion of DNA. Students will perform PCR amplification reactions of several DNA samples. After checking the amplification products on an agarose gel, the remaining sample will be digested with a restriction enzyme. When the digested samples are analyzed on an agarose gel, students will identify different polymorphic genes.