DNA Fingerprinting: Southern Blot Analysis (Non-Isotopic Detection)

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Price: $499 + GST
Model Number: 311


For 5 Groups.

Electrophoresis - 45 min. Blotting - overnight. Non-Isotopic Detection 3-4 hrs.

In this experiment, students gain experience in non-isotopic DNA detection & the use of Southern Blot analysis in DNA fingerprinting for a hypothetical paternity test. Includes three modules: agarose gel electrophoresis, Southern Blot transfer, and non-isotopic detection of DNA.

Kit includes:

  • Instructions
  • Predigested DNA samples
  • Buffers
  • NBT/BCIP tablets
  • Streptavidin-Alkaline Phosphatase
  • Nylon membranes
  • Filter paper
  • UltraSpec-Agarose™ powder.

Requirements:

  • Electrophoresis apparatus
  • Power supply
  • Automatic micro-pipette with tips
  • Waterbath
  • Incubation oven,