Purification of the Restriction Enzyme Eco RI
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Price: $478 + GST
Model Number: 302
For 5 Purifications.
Packing column 45 minutes.
Restriction analysis A 35 minutes.
Restriction analysis B 50 minutes.
Gel Prep 30 minutes.
Electrophoresis 30 minutes.
Staining & Destaining 2 minutes.
In this experiment, students actually purify the restriction enzyme, Eco RI! This procedure utilizes an ion exchange chromatography step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by agarose gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated.
Kit includes:
- Instructions
- Ion exchange matrix
- Chromatography columns
- E.coli cell extract
- Equilibration & elution buffer
- Lambda DNA
- Lambda/Eco RI Marker
- KCl
- Glycerol
- Dilution & reaction buffers
- Gel loading solution
- Agarose
- Electrophoresis buffer
- Stain.
Requirements:
- Horizontal gel electrophoresis apparatus
- Power supply
- UV transilluminator
- Waterbath
- Microcentrifuge
- UV spectrophotometer & cuvettes
- Automatic micropipet with tips
- Ring stands & clamps
- Ice and ice buckets.
